TABLE 2.
Derivatives accumulated upon biotransformation of p-coumaric acid by whole cells of LPNC8 and the LPD1 mutant, induced with p-coumaric or ferulic acid
| Strain | Derivative(s) present after inductiona by:
|
|||||||
|---|---|---|---|---|---|---|---|---|
|
p-Coumaric acid
|
Ferulic acid
|
|||||||
| 1.2 mM
|
3 mM
|
1.2 mM
|
3 mM
|
|||||
| − | + | − | + | − | + | − | + | |
| LPNC8 | 4VP | 4VP | 4VP | 4VP | 4VP | 4VP | 4VP | 4VP |
| LPD1 | RD | RD | 4VP/RD | RD | 4VP | RD | 4VP | 4VP/RD |
a
Whole cells were incubated for 15 min at 30°C with (+) or without (−) 20 mM glucose before kinetic reactions. 4VP, 4-vinyl phenol; RD, reduced derivatives, i.e., phloretic acid and/or 4-ethyl phenol; 4VP/RD, mixture of 4-vinyl phenol and reduced derivatives.