TABLE 3.
Derivatives from the biotransformation of ferulic acid by whole cells of LPNC8 and the LPD1 mutant, induced with p-coumaric or ferulic acid
| Strains | Derivative(s) present after inductiona by:
|
|||||||
|---|---|---|---|---|---|---|---|---|
|
p-Coumaric acid
|
Ferulic acid
|
|||||||
| 1.2 mM
|
3 mM
|
1.2 mM
|
3 mM
|
|||||
| − | + | − | + | − | + | − | + | |
| LPNC8 | 4VG | 4VG/RD | 4VG | 4VG/RD | 4VG | 4VG/RD | 4VG | 4VG |
| LPD1 | RD | RD | 4VG/RD | RD | 4VG | RD | 4VG | 4VG/RD |
Whole cells were incubated for 15 min at 30°C with (+) or without (−) 20 mM glucose before kinetic reactions. 4VG, 4-vinyl gaïacol; RD, reduced derivatives, i.e., dihydroferulic acid and/or 4-ethyl gaïacol; 4VG/RD, mixture of 4-vinyl gaïacol and reduced derivatives.