Fig. 4.

Metabolic labeling of proteins to determine temperature dependence of global protein translation in cell lines. (A) Linearity of method for quantifying incorporation of L-homopropargylglycine (HPG) into protein in live cells via CLICK chemistry and fluorescence quantification of labeled protein following PAGE. Simple linear regression r² = 0.0996, p < 0.0001. (B) Temperature dependence of relative rates of global translation in meadow jumping mouse and lab mouse cells (n = 3 technical replicates per species cell line). Significant species differences in translation rates at each time point (two-sided t-test) are denoted *.