Fig. 6.

Consistent physiological responses are observed across passage number in mammalian primary fibroblasts. (A) Heat shock 70 induction in 13-lined ground squirrel primary fibroblast exposed to 24 hours at 41 °C compared to 37 °C baseline. (B) Paired comparison of HSP70 induction in early passage (P4) versus mid-passage (P10) 13-lined ground squirrel fibroblasts (n = 3 individuals) after 24 hour exposure to 41 °C (two-sided t = 1.118, df = 2, p = 0.38). (C) Oxygen consumption rate (OCR) of Southern white rhinoceros primary fibroblasts did not differ across passage number (n = 3 individuals for Early passage (P4,5,6), n = 5 paired samples in Mid (P9,10,12) and Late (P15,16,17,19) passages; Mixed effect model F1,5 = 3.46, P = 0.12).