TABLE 2.
Specific substrate consumption rates and product yields for xylose and glucose fermentation at different steady statesa
| Feed denomination (g/liter) | Substrate consumption rate (mmol of C h−1 g [dry wt] of cells−1)
|
Product yield (mmol of C of product/mmol of C of total carbohydrates)
|
% C recoverye | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Xylose | Glucose | EtOH | EtOHb (corrected) | EtOHc (on glucose) | XOH | XOHd (on xylose) | Acet | Glyc | Biomass | CO2 | ||
| 15 xylose + 5 glucose | −6.8 | −15.1 | 0.34 | 0.45 | 0.65 | 0.12 | 0.39 | 0.006 | 0.078 | 0.11 | 0.23 | 98.2 |
| 10 xylose + 10 glucose | −4.7 | −18.0 | 0.38 | 0.46 | 0.58 | 0.09 | 0.42 | 0.004 | 0.090 | 0.11 | 0.27 | 101.1 |
| 5 xylose + 15 glucose | −3.3 | −22.7 | 0.37 | 0.50 | 0.58 | 0.03 | 0.24 | 0.004 | 0.094 | 0.09 | 0.26 | 98.6 |
| 0 xylose + 20 glucose | 0.0 | −23.8 | 0.39 | 0.53 | 0.53 | 0.004 | 0.085 | 0.10 | 0.29 | 100.6 | ||
The total carbon concentration in the feed was 667 mmol of C/liter, or 20 g/liter. EtOH, ethanol; XOH, xylitol; Acet, acetate; Glyc, glycerol.
Ethanol yield was corrected by assuming that the missing percentage in the degree of reduction balance was due to evaporated ethanol.
Ethanol yield was calculated based on consumed glucose only.
Xylitol yield was calculated based on xylose only.
C recovery includes the product yields calculated for total carbohydrates.