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. Author manuscript; available in PMC: 2023 Jun 6.
Published in final edited form as: Curr Biol. 2022 May 23;32(11):2563–2571.e4. doi: 10.1016/j.cub.2022.04.046

Key resources table.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit anti-KNL-3 6 N/A
Rabbit anti-KNL-1 40 N/A
Rabbit anti-NDC-80 40 N/A
Rabbit anti-HCP-4 41 N/A
Bacterial and virus strains
E. coli Strain: OP50-1 Caenorhabditis Genetics Center (CGC) N/A
Experimental models: Organisms/strains
C. elegans: Strain wild type N2 (ancestral) CGC N2
C. elegans: Strain OD3070 ltIs37 [pAA64; pie-1/mCHERRY::his-58; unc-119 (+)] IV; knl-3(lt46 [gfp::knl-3]) V This Study OD3070
C. elegans: Strain OD4492 mel-28(ax640) III; ltIs37 [pAA64; pie- 1/mCHERRY::his-58; unc-119 (+)] IV; knl-3(lt46 [gfp::knl-3]) V This Study OD4492
C. elegans: Strain GKC4 mel-28(ax640) III 9 GKC4
C. elegans: Strain OD868 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I This Study OD868
C. elegans: Strain OD4443 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; mel-28(ax 640) III This Study OD4443
C. elegans: Strain OD3796 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; emb-30(tn377) III This Study OD3796
C. elegans: Strain OD2388 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc- 119(+)]I;ltSi1[pOD809/pJE110; Pknl-1::KNL-1reencoded::RFP; cb-unc-119(+)]II; unc-119(ed3)III? This Study OD2388
C. elegans: Strain OD3365 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; ltSi14[pOD857/pJE132; Pknl-1::KNL-1reencoded(RRASA + SAAA mutant)::RFP; cb-unc-119(+)]II This Study OD3365
C. elegans: Strain OD4573 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; ltSi120[[pDC170;Pndc-80:NDC-80 reencoded; cb-unc-119(+)]II; mel-28(ax640) III This Study OD4573
C. elegans: Strain OD4493 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; ltSi711[pDC267;Pndc-80:NDC-80(66,96,100,125,144,155AAAAAA) reencoded; cb-unc-119(+)] II; mel-28(ax640) III This Study OD4493
C. elegans: Strain OD1500 ltSi466[pNH97; Pmel-28::GFP-mel-28 (reencoded exon 14 FL); cb-unc-119(+)] II; ltIs37 [pAA64; pie-1/mCHERRY::his-58; unc-119 (+)] IV This Study OD1500
C. elegans: Strain OD1861 ltSi596[pNH118; Pmel-28::GFP-mel-28 1-1559 (reencoded exon 14 FL); cb-unc-119(+)] II; ltIs37 [pAA64; pie-1/mCHERRY::his-58; unc-119 (+)] IV This Study OD1861
C. elegans: Strain OD4444 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; ltSi700[pNH164; Pmel-28::mel-28rr ex14 WT; cb-unc-119(+)] II; mel-28(ax640) III This Study OD4444
C. elegans: Strain OD4446 ltSi220[pOD1249/pSW077; Pmex-5::GFP-tbb-2-operon-linker-mCherry-his-11; cb-unc-119(+)] I; ltSi1119[pNH286; untagged mel-28 11559 RRexon14::mel-28 3’UTR; cb-unc-119(+)]II; mel-28(ax640) III This Study OD4446
C. elegans: Strain FM348 aspm-1(or1935[GFP::aspm-1]) I; ltIs37[pAA64; pie-1/mCHERRY::his-58; unc-119 (+)] IV 17 FM348
C. elegans: Strain OD3192 klp-19(lt118[gfp::klp-19]) III; unc-119(ed3) III?; ltIs37[pAA64;pie-1/mCHERRY::his58; unc-119(+)] IV This Study OD3192
Oligonucleotides
Primer 1 for synthesis of dsRNA targeting mel-28(C38D4.3): taatacgactcactataggAGAAATACTCGATCCGCATC; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting mel-28(C38D4.3): aattaaccctcactaaaggAGTTACACTTCCAGCTCTAC; Template: N2 Genomic DNA This Study N/A
Primer 1 for synthesis of dsRNA targeting mel-28 (C38D4.3) for KLP-19 localization analysis: aattaaccctcactaaaggCGTGTCCTGGATGTTCT GG; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting mel-28 (C38D4.3) for KLP-19 localization analysis: taatacgactcactataggTTTATCGATGGGAGGCATTC; Template: N2 Genomic DNA This Study N/A
Primer 1 for synthesis of dsRNA targeting hcp-4(T03F1.9): aattaaccctcactaaaggGGAAATGTACGGAGCGAAAA; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting hcp-4(T03F1.9): taatacgactcactataggACATTGTTGGTGGGTCCAAT; Template: N2 Genomic DNA This Study N/A
Primer 1 for synthesis of dsRNA targeting knl-1(C02F5.1): taatacgactcactataggAACAACCATGTGAGGCTGGT; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting knl-1(C02F5.1): aattaaccctcactaaaggGCGCCAGTATTCGAGGTATC; Template: N2 Genomic DNA This Study N/A
Primer 1 for synthesis of dsRNA targeting ndc-80(W01B6.9): aattaaccctcactaaaggGATGACAAGTACATTCAGAGATTATACAAATGATC; Template: N2 cDNA This Study N/A
Primer 2 for synthesis of dsRNA targeting ndc-80(W01B6.9): taatacgactcactataggGTGGTTCAAGATTCATTTGAATATTAAGTCCACTG; Template: N2 cDNA This Study N/A
Primer 1 for synthesis of dsRNA targeting rod-1(F55G1.4): aattaaccctcactaaaggAATGCAAATCTTTTTGGATGGGAGAAAC; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting rod-1(F55G1.4): taatacgactcactataggCATCGACGAATTTGATTCGATCAATC; Template: N2 Genomic DNA This Study N/A
Primer 1 for synthesis of dsRNA targeting knl-3(T10B5.6): taatacgactcactataggGTCGAGAAAACTTCCGTGAAG; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting knl-3(T10B5.6): aattaaccctcactaaaggATGTCTCAAAAATCAAACGACACC; Template: N2 Genomic DNA This Study N/A
Primer 1 for synthesis of dsRNA targeting npp-6 (F56A3.3): taatacgactcactataggATTGTCGTTCCCACAGCTTC; Template: N2 Genomic DNA This Study N/A
Primer 2 for synthesis of dsRNA targeting npp-6 (F56A3.3): aattaaccctcactaaaggTATTGCCGATCGGTTCTTCT; Template: N2 Genomic DNA This Study N/A
Recombinant DNA
Plasmid for GFP::KLP-19 CRISPR Tagging: pDD162 44 Addgene_47549
Plasmid for GFP::KLP-19 CRISPR Tagging: pCFJ90 [Pmyo-2::mCherry (pharynx muscle)] 45 Addgene_19327
Plasmid for GFP::KLP-19 CRISPR Tagging: pCFJ104 [Pmyo-3::mCherry (body muscle)] 45 Addgene_19328
Plasmid for GFP::KLP-19 CRISPR Tagging: pGH8 [Prab-3::mCherry (pan-neuronal)] 45 Addgene_19359
Software and algorithms
Fiji 43 SCR_002285
Prism GraphPad SCR_002798
Adobe Illustrator Adobe SCR_010279
Other