TABLE 1.
Culture parameters, elemental growth yields, elemental cellular composition, cellular PHA content, and PHA composition during growth of P. oleovorans in continuous culture at different dilution rates with octanoate and ammonium under either carbon- or nitrogen-limited conditions
| Parameter | Abbrev.a | Unit | Dilution rate or specific growth rateb
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.05 h−1
|
0.10 h−1
|
0.20 h−1
|
0.30 h−1
|
0.40 h−1
|
μmax (h−1) (batch)c | ||||||||
| C-lim. | N-lim. | C-lim. | N-lim. | C-lim. | N-lim. | C-lim. | N-lim. | C-lim. | N-lim. | ||||
| Observed C/N borders | C0/N0 | mol mol−1 | 11.6 | —d | 10.6 | 20.4 | 8.3 | 17.4 | 6.8 | 13.5 | 7.5 | 9.1 | — |
| Calculated C/N borderse | C0/N0 | mol mol−1 | 12.0 | — | 9.8 | 24.0 | 7.8 | 18.0 | 6.7 | 13.9 | 6.7 | 9.9 | 6.9 |
| Carbon-based growth yield | YX/C | g g−1 | 0.62 | — | 0.83 | 0.65 | 1.07 | 0.8 | 1.19 | 0.86 | 1.27 | 0.97 | 1.23 |
| Nitrogen-based growth yield | YX/N | g g−1 | 6.4 | — | 6.94 | 13.36 | 7.15 | 12.37 | 6.85 | 10.26 | 7.29 | 8.28 | 7.30 |
| Specific carbon consumption ratef | qs | g (g · h)−1 | 0.08 | — | 0.12 | 0.15 | 0.19 | 0.25 | 0.25 | 0.35 | 0.31 | 0.41 | 0.39 |
| Cellular PHA content | wt% | 1.6 | — | 1.4 | 56.1 | 2.2 | 42.3 | 10.1 | 31.2 | 7.6 | 20.9 | — | |
| Specific PHA accumulation rateg | qPHA | g (g · h)−1 | 0.001 | — | 0.001 | 0.133 | 0.005 | 0.147 | 0.038 | 0.137 | 0.035 | 0.108 | 0.031 |
| PHA yield coefficienth | YPHA/C | g g−1 | 0.01 | — | 0.01 | 0.25 | 0.02 | 0.23 | 0.09 | 0.18 | 0.07 | 0.15 | 0.046 |
| Cellular C content | wt% | 48.7 | — | 49.0 | 57.4 | 48.7 | 54.6 | 48.9 | 52.3 | 48.5 | 49.6 | — | |
| Cellular N content | wt% | 12.7 | — | 12.7 | 5.8 | 12.5 | 7.3 | 11.7 | 8.9 | 12.1 | 10.8 | — | |
| Cellular H content | wt% | 7.1 | — | 7.2 | 8.0 | 7.1 | 7.3 | 6.8 | 7.7 | 7.1 | 7.4 | — | |
| C/N ratio of dry cell mass | mol mol−1 | 4.5 | — | 4.5 | 11.6 | 4.4 | 8.7 | 4.9 | 6.8 | 4.7 | 5.4 | — | |
| C/N ratio of PHA-free biomass | mol mol−1 | 4.4 | — | 4.4 | 3.8 | 4.3 | 4.2 | 4.1 | 4.1 | 4.2 | 3.8 | — | |
a
Abbrev., abbreviation.
b
C-lim., carbon-limited growth; N-lim., nitrogen-limited growth.
c
The maximum growth rate (μmax) for P. oleovorans with octanoate was 0.48 h−1; data are taken from reference 12.
d
—, calculation or analysis not possible.
e
Calculation according to reference 13.
f
Grams of carbon used per gram of CDW per hour.
g
Grams of PHA accumulated per gram of PHA-free biomass per hour.
h
Grams of carbon accumulated in PHA per gram of carbon used.