Table 1.
Primer sequences for amplification of 18SrRNA gene, pfhrp2 and pfhrp3 genes in malaria parasites
| Species | Primer | Sequence (5’-3’) | Expected fragment length in Base pairs (bp) | |
|---|---|---|---|---|
| Plasmodium spp. | rPLU5 | CCTGTTGTTGCCTTAAACTTC | 900 | |
| rPLU6 | TTAAAATTGTTGCAGTTAAAACG | |||
| P. falciparum | rFAL1 | TTAAACTGGTTTGGGAAAACCAAATATATT | 205 | |
| rFAL2 | ACACAATGAACTCAATCATGACTACCCGTC | |||
| pfhrp2 | Outer | Pfhrp2-F1 | CAAAAGGACTTAATTTAAATAAGAG | 600 – 1000 |
| Pfhrp2-R1 | AATAAATTTAATGGCGTAGGCA | |||
| Nested | Pfhrp2-F2 | ATTATTACACGAAACTCAAGCAC | ||
| Pfhrp2-R1 | AATAAATTTAATGGCGTAGGCA | |||
| pfhrp3 | Outer | Pfhrp3-F1 | AATGCAAAAGGACTTAATTC | 600 – 950 |
| Pfhrp3-R1 | TGG TGTAAGTGATGCGTAGT | |||
| Nested | Pfhrp3-F2 | AAATAAGAGATTATTACACGAAAG | ||
| Pfhrp3-R1 | TGG TGTAAGTGATGCGTAGT | |||