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Thermocycling conditions for amplification of Plasmodium falciparum
| Cycling Conditions |
|||
|---|---|---|---|
| Steps | Temperature |
Time | |
| Primary PCR | Nested PCR | ||
| Pre-denaturation | 94°C | 3 minutes | |
| Denaturation | 94°C | 1 minute | |
| Annealing | 55°C | 61°C | 1 minute |
| Extension | 68°C | 1 minute | |
| Number of Cycles | 25 | 30 | |
| Final extension | 68°C | 5 minutes | |
| Hold | 4°C | ∞ | |
