Table 3.

Thermocycling conditions for amplification of Pfhrp2 and Pfhrp3 Genes

Species		Initial Denaturation	Denaturation	Annealing	Extension	Cycles	Final extension	Hold
Pfhrp2	Outer	94° for 5 minutes	94°C for 30 seconds	59°C for 1 minute	68°C for 1 minute	40	68°C for 3 minutes	4°C
	Nested	94°C for 5 minutes	94°C for 30 seconds	59°C for 1 minute	68°C for 1 minute	40	68°C for 3 minutes	4°C
Pfhrp3	Outer	94°C for 5 minutes	94°C for 30 seconds	55°C for 1 minute	68°C for 1 minute	40	68°C for 3 minutes	4°C
	Nested	94°C for 5 minutes	94°C for 30 seconds	55°C for 1 minute	68°C for 1 minute	40	68°C for 3 minutes	4°C