Figure 2.

Generation of camelid single-domain antibodies [nanobodies or heavy-chain variable domain (VHH)]. Peripheral blood lymphocytes (PBLs) are collected following two or three rounds of immunizations. Purified PBLs are used to generate VHH phage-display libraries. Target specific nanobodies are then selected from the generated library after two or three rounds of panning. Nanobodies, expressed with appropriate tags (e.g. a sortase tag and/or a His-6 tag), are then ready for the installation of radiometal chelators or click handles.