Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(A) Direct radiolabeling of His-tagged (H6) nanobodies with 99mTc-tricarbonyl.76 (B) Site-specific labeling of nanobodies using sortase. A nanobody equipped with an LPXTG tag can be labeled with a triglycine-containing sortase substrate. X can be a number of amino acids, such as E. A His-tag (H6) can be used to purify the protein. R can be any biomolecule of interest. Sortase reaction yields near-complete formation of product.77,78
