Figure 2.
Comparison of staining efficiency before and after oligo conjugation of antibodies. (A) Standard immunohistochemical staining for CD16 and CD39 before and after oligo conjugation of the antibodies. Rabbit immunoglogulin G (IgG) was used as the background control. Scale bar 100 μm. (B) Housekeeping (HK)-normalized digital spatial profiling counts after oligo conjugation of antibodies from three discrete regions of interest (ROI1–3). Violet bars, rabbit IgG; pink bars (upper figure), CD16; pink bars (lower figure), CD39.
