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. 2019 May 30;1:11–18. doi: 10.1016/j.iotech.2019.05.001

Figure 3.

Figure 3

Selection of type of region of interest (ROI). Three types of regions can be selected within the digital spatial profiling platform. Tissue biopsy was stained with S100B/PMEL17 (green), LDH (red) and CD45 (yellow) visualization markers. (A–C) Geometric ROIs can be selected, ranging from circles (A) to rectangles (B) to user-defined polygons (C). (D,E) Segmentation within a geometric ROI is generated based on visualization markers, and can currently distinguish between tumor and stroma (D) or specific cell type populations (E). (F) Single-cell ROIs are generated based on visualization markers which allow the analysis of either one or multiple single cells within a field of view. Scale bar 100 μm.