Table 1.
Overview of (A) visualization markers used currently to distinguish between the tumor and immune cell compartment, and (B) digital spatial profiling protein probes included in the core panel and the additional modules
| A | |
|---|---|
| Visualization markers | Compartment |
| Syto13 | DNA |
| S100B/Pmel17 or PanCK | Tumor |
| CD45 | Immune cell |
| CD3 | Immune cell |
| B | |||
|---|---|---|---|
| Core panel | Module 1 | Module 2 | |
| Beta-2-microglobulin | CD56 | CD137 | CD127 |
| CD11c | HLA-DR | LAG3 | CD25 |
| CD20 | SMA | OX40L | CD80 |
| CD3 | Fibronectin | TIM-3 | CD86 |
| CD4 | TGFB1 | VISTA | ICOS |
| CD45 | PD-L1 | ARG1 | PD-L2 |
| CD68 | GZMB | B7-H3 | CD40 |
| CD8 | Ki-67 | IDO1 | CD40L |
| CTLA4 | PD1 | STING | CD27 |
| PanCK | IgGs | GITR | CD44 |