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Neurotransmitter preparation (top) and experimental design (bottom): the flatworms were fasted for 14 days, and then presented with the drug-infused egg yolk, and immersed in drug-treated water, for 40 minutes prior to the experiment (“soak and yolk”). Animals were transferred to spring water for the duration of the recording (40 minutes); the initial 10 minutes were excluded from analysis owing to acclimation. Created with BioRender.com.
