TABLE 2.
EC50s and MICs of DMIs and other toxicants for the transformants into which PdCYP51 was introduced
| Toxicant | Result with the indicated strain (μg/ml)
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| PD5
|
PD5 (PdCYP51-P)-21
|
PD5 (PdCYP51-L)-7
|
PD5 (PdCYP51-L)-15
|
LC2
|
||||||
| EC50 | MIC | EC50 | MIC | EC50 | MIC | EC50 | MIC | EC50 | MIC | |
| Triflumizole | 0.05 ± 0.02 | 0.5 | 0.15 ± 0.02 | 5 | 1.6 ± 0.9 | >100 | 1.6 ± 0.8 | >100 | 2.5 ± 0.7 | >100 |
| Fenarimol | 0.11 ± 0.03 | 1 | 0.25 ± 0.12 | 10 | 0.64 ± 0.14 | 100 | 0.95 ± 0.18 | >100 | 1.9 ± 0.3 | >100 |
| Bitertanol | 0.14 ± 0.04 | 5 | 0.35 ± 0.03 | 10 | 1.2 ± 0.3 | >100 | 1.6 ± 0.2 | >100 | 2.6 ± 1.6 | >100 |
| Pyrifenox | 0.03 ± 0.00 | 5 | 0.28 ± 0.17 | 10 | 1.5 ± 0.3 | 100 | 3.1 ± 1.6 | >100 | 42 ± 12 | >100 |
| Cycloheximide | 0.11 ± 0.02 | 1 | 0.11 ± 0.03 | 1 | 0.08 ± 0.03 | 1 | 0.07 ± 0.02 | 1 | 0.21 ± 0.07 | 5 |
| 4NQO | 0.37 ± 0.02 | 5 | 0.33 ± 0.17 | 5 | 0.35 ± 0.16 | 5 | 0.29 ± 0.16 | 5 | 0.75 ± 0.52 | 10 |
| Acriflavine | 0.58 ± 0.24 | 5 | 0.60 ± 0.08 | 5 | 0.52 ± 0.08 | 5 | 0.77 ± 0.17 | 5 | 2.8 ± 1.2 | >100 |