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. 2022 Jun 22;8(25):eabn9699. doi: 10.1126/sciadv.abn9699

Table 1. Summary of integrated molecular characteristics of HCC.

Summary of integrated landscape from genetic, transcriptomic and metabolomics platforms in HCC tumors.

C1 C2 C3 C4
Samples 6/28 (21%) 6/28 (21%) 7/28 (25%) 9/28 (32%)
HMIN 5/6 (83%) 2/6 (33%) 3/7 (43%) 3/9 (33%)
HWIDE 1/6 (17%) 4/6 (67%) 4/7 (57%) 6/9 (67%)
Recurrence 0/6 (0%) 0/6 (0%) 2/7 (29%) 4/9 (44%)
mTOR pathway 5/6 (83%) 4/6 (67%) 6/7 (86%) 7/9 (78%)
mtDNA 4/6 (67%) 5/6 (83%) 4/7 (57%) 5/9 (56%)
LOH/UPD 0/6 (0%) 4/6 (67%) 5/7 (71%) 4/9 (44%)
Gene expression pathway Upregulation of genes in the
TCA cycle
Upregulation of MYC target
pathway, downregulation of
genes in the TCA cycle
Upregulation of oxidative
phosphorylation
Upregulation of
inflammation and the
immune response
Metabolic pathway Enriched lysine degradation Elevation of ceramide/
sphingomyelin species
Enriched valine, leucine and
isoleucine biosynthesis, and
lysine degradation
Elevation of acylcarnitine
species, kynurenine, and
1-methylnicotinamide,
depletion of saccharopine
and 3 constituents of the
NAD+ pathway
TME signatures Depletion of CD8+ T cells and
elevation of PDL1
Depletion of Th cells and
elevation of PDL1 and other
immune checkpoints
Depletion of Th cells Enriched regulatory T cells,
exhausted T cells and
depletion of Th17 cells
Gene expression Upregulation of NEAT1,
MALAT1
Upregulation of IDO1,
chemokine ligand family
members

Abbreviations: HCC, Hürthle cell carcinoma; C, cluster; HMIN, minimally invasive HCC; HWIDE, widely invasive HCC; mTOR, mechanistic target of rapamycin; mtDNA, mitochondrial DNA; LOH/UPD, loss of heterozygosity/uniparental disomy; MYC, MYC proto-oncogene, bHLH transcription factor; TCA, citric acid cycle; NAD, nicotinamide adenine dinucleotide; TME, tumor microenvironment; CD8, cluster of differentiation 8; PDL1, programmed death-ligand 1; Th, T helper