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. 2022 Jun 9;18(6):e1010223. doi: 10.1371/journal.pgen.1010223

Fig 4. The unc-85 mutation reduces chromatin H3.

Fig 4

(A) Fluorescence time-lapse images (up) and heatmaps (down) of endogenously GFP-tagged HIS-74 (H3) with the mCherry-tagged (magenta) membrane during one-cell stage C. elegans embryo in WT (left) or H2B 3KO; unc-85(cas1100) quadruple mutant (right) embryos. Scale bar, 5 μm. (B) Quantifications of the whole-cell GFP-tagged HIS-74 fluorescence intensity (left) and intensity ratio of the nuclear to the cytoplasm (right). Data are presented as mean ± SD (error bars), N = 21–34. Statistical significance is based on two-way ANOVA, n.s., not significant, ***p < 0.001. (C) Heat maps show the signals of H3 flanking (± 3 kb) around the gene bodies. (D) Average enrichment profile of H3 reads flanking (± 3 kb) around the gene bodies; the y-axis represents the average normalized number of fragments at the corresponding genomic regions indicated in the x-axis. (E) Genome tracks show H3 signals of a representative genomic locus (Chr IV: 10,627,008–10,840,892) in WT (up) or H2B 3KO; unc-85(cas1100) quadruple mutants (middle). Heatmap shows H3 depletion of H2B 3KO; unc-85(cas1100) quadruple mutant relative to WT (bottom). Dark blue boxes show the reference genes.