TABLE 1.
Bacteria and plasmids used in this study
Strain or plasmid | Source or reference | Comments |
---|---|---|
E. coli DH5α | American Type Culture Collection | supE44 ΔlacU169 (φ80 lacZ ΔM15)hsd R17 recA1 gyrA96 thi-1 relA1 (16) |
E. coli S17-1λpir | American Type Culture Collection | RP4; pir lysogen (15) |
B. diminuta ATCC 19146 | Pall Europe Limited | Filter challenge test strain (2) |
pSfi390 | S. Swift | pUC18 (16) containing lux genes from P. luminescens ATCC 29999 (13) |
pUTLUXAB | G. Stewart | RP4 oriT, R6K plasmid, Tn5::tetracycline resistance cassette containing luxAB genes from Vibrio harveyi (6) |
pVAGFP | S. Taylor | Plasmid pVAGFP containing mutant gene for GFP (5) |
pBSLLUX2 | This study | pBSL204 with luxABCDE genes (13) |
pBSLGFP1 | This study | pBSL204 with gfp gene (5) |
pBSL204 | Department of Molecular Cell Biology, Utrecht, The Netherlands | RP4 oriT, R6K plasmid, Tn5::tetracycline resistance cassette (1) |