TABLE 3.
Nightowl system detection sensitivity at various scan parametersa
Background subtraction | Scan time (min) | No. of microcolonies detected after 24-h incubationb | Sensitivity (%) |
---|---|---|---|
No | 1 | 3, 4, 7, 8, 9, 18 | 71.9 |
Yes | 1 | 3, 4, 7, 8, 9, 18 | 71.9 |
Yes | 60 | 3, 3, 4, 7, 5, 12 | 54.3 |
No | 60 | 3, 3, 4, 7, 5, 12 | 54.3 |
The colony counts after 24 h of incubation as detected by the Nightowl system (pixel setting, 9 by 9) are shown. The colony counts after 48 h of incubation for each of the six membrane samples were 4, 4, 8, 10, 26, and 33 CFU.
A result is given for each of the six membranes.