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. 2000 Aug;66(8):3432–3437. doi: 10.1128/aem.66.8.3432-3437.2000

TABLE 4.

Detection sensitivities for all detection methods used

Method No. of visible colonies at 48 h % Sensitivity at 24 h
Epifluorescence microscopya 76 94.7
58 100
43 93.0
39 94.9
35 97.1
29 96.6
23 100
21 100
19 100
18 100
15 100
8 100
7 85.7
5 100
4 100
3 100
2 100
2 100
1 100
1 100
Nucleovisionb 66 53.0
49 65.3
40 92.5
35 85.7
3 42.4
24 45.8
21 57.1
16 87.5
15 80.0
14 92.9
11 90.9
10 30.0
10 100
10 90.0
7 100
6 100
5 80.0
4 100
2 50.0
2 100
2 100
1 100
1 100
Nightowl (ae3)c 66 77.3
57 73.7
46 84.8
26 34.6
22 72.7
15 100
10 50.0
10 90.0
9 88.9
8 87.5
4 100
4 75.0
Nightowl (ab5)d 71 81.7
64 81.3
33 51.5
25 84.0
25 100
19 100
12 75.0
12 100
6 83.3
4 100
2 100
2 50
a

Mean sensitivity, 98.1%. 

b

Nucleovision CCD camera (strain ab5). Mean sensitivity, 80.1%. 

c

Nightowl CCD camera (strain ae3). Mean sensitivity, 77.0%. 

d

Nightowl CCD camera (strain ab5). Mean sensitivity, 83.9%.