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Reaction rates (expressed as initial reaction velocity V0 normalized to the concentration of enzyme Et) for the cleavage of SARS-CoV-2 spike S1/S2 ancestral (P681) and variant (P681H and P681R) peptide substrates by (A) factor Xa, (B) furin, and (C) TMPRSS2, and (D) Thrombin were measured over a range of 0–80 μM substrate.
