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Relative activity of factor Xa (125nM) with or without 10μM nafamostat in reaction with S1/S2 FRET peptide substrate (50 μM) corresponding to (A) WT ancestral sequence P681, (B) P681H substitution, and (C) P681R substitution. * P<0.05, two-tailed t-test. Error bars represent +/− SEM.
