TABLE 2.
Primers developed for PCR and sequencing of recA genes
| Primer | Nucleotide (amino acid) positiona | Primer sequence |
|---|---|---|
| GPRA-FBb | 289–305 (96–101) | Glu His Ala Leu Asp Pro |
| 5′-GAR CAY GCN CTN GAY CC-3′ | ||
| Pro | ||
| GPRA-R2 | 638–619 (212–206) | Gly Gly Thr Thr Thr Glu Pro |
| 5′-CC SCC SGK SGT SGT YTC NGG-3′ | ||
| Ser | ||
| GPRA-UF2 | 67–86 (22–28) | Gly Lys Gly Ala Val Met Arg |
| 5′-GGS AAG GGS KCN GTN ATG CG-3′ | ||
| Ala Phe Lys | ||
| GPRA-UR2 | 910–893 (304–298) | Glu Lys Gly Gln Gly Leu Gln |
| 5′- C CTT SCC CTG SCC NAR YT-3′ | ||
| A19-F2 | 79–98 | 5′-GTCATGCGCCTGGGCGACGA-3′ |
| A1-R1 | 909–890 | 5′-CTTGCCCTGGCCGAGTTGGT-3′ |
| AU-F1 | 120–137 | 5′-CATYCCCACCGGHTCCAT-3′ |
| AU-R1 | 834–817 | 5′-CATGTCGATGATGCCGCC-3′ |
| AU-FM1 | 463–482 | 5′-GAAATCGAAGGCGACATGGG-3′ |
| AU-RM1 | 531–514 | 5′-ACGCAGGGCCTGGCTCAT-3′ |
a
Position relative to that in E. coli recA (A of initial ATG = position 1; amino acid numbering started after initial Met).
b
Primers identical to GPRA-FB and similar to GPRA-R2 (adapted here for the high G+C content of Arthrobacter) were used previously (8) to amplify recA genes from various low-G+C gram-positive bacteria.