Figure 6.

Antiviral activity of LabMol-309. The EC₅₀ (A) and CC₅₀ (B) curves from replicon-based assays are shown. CHIKV replicon cells were incubated with the compound at twofold serial dilutions (from 20 µM to 0.03 µM for EC₅₀ and from 100 to 0.3 µM for CC₅₀) for 48 h, and Gluc activity/cell viability were measured from cells' supernatant. For the CHIKV-nanoluc replication assay evaluation of EC₅₀ and CC₅₀ (C), BHK-21 cells were treated with concentrations of LabMol-309 ranging from 0.78 to 100 µM, in the presence or absence of CHIKV-nanoluc for 16 h, and viral replication was quantified by measuring the nanoluciferase activity (indicated by ●) and cellular viability was measured using an MTT assay (indicated by ^▄). Representative results from two independent experiments performed in duplicates. Error bars represent the standard deviations. Figures and statistical analysis were performed using GraphPad Prism 8.