Table 2.
Thermodynamic parameters associated with the nsP4-CHIKV thermal denaturation by DSC.
| Concentration (µM) | Rate (°C/h) | Tm (°C) | ΔHcal (kcal/mol) | ΔT1/2 (°C) | ΔS (cal/mol) | ΔHvH (kcal/mol) |
|---|---|---|---|---|---|---|
| 9.3 | 8 | 36.3 | 205 | 4.8 | 662 | 142 |
| 11.9 | 13 | 37.0 | 102 | 4.4 | 330 | 151 |
| 7.4 | 33 | 38.8 | 86 | 4.3 | 275 | 165 |
| 7.4 | 64 | 39.8 | 66 | 4.5 | 210 | 150 |
Tm represents the temperature where CP reaches its maximum value. ΔHcal was calculated as the area under the DSC trace. ΔT1/2 corresponds to the linewidth at half the height of the transition peak. The entropy change at Tm, ΔS, was calculated as ΔS = ΔHcal/Tm. ΔHvH was calculated as 4RTm2Cp,max/ΔHcal. Analyses of the thermograms were performed with MicroCal Origin software.
Uncertainties: Tm (± 0.2 °C), ΔHcal (± 1‒3 kcal/mol), ΔT1/2 (± 0.2 °C).