| Gene (Homo sapiens) |
ERN1 |
HUGO Gene Nomenclature Committee |
HGNC:3,449 |
Gene encoding human IRE1a |
| Strain, strain background (Escherichia coli) |
Stellar Competent Cells |
Takara Bio |
636,763 |
High-efficiency competent cells for cloning |
| Cell line (Homo-sapiens) |
U-2 OS WT Flp-In T-REx |
Ivan Dikic lab; verified by ATCC STR service; published in Belyy et al., 2020
|
PWM253 |
Parental line for all cells in this study, denoted as “WT” |
| Cell line (Homo-sapiens) |
U-2 OS IRE1α KO |
Belyy et al., 2020
|
PWM254 |
CRISPR knock-out of IRE1α in PWM253 |
| Cell line (Homo-sapiens) |
U-2 OS IRE1α partial KO |
This paper |
PWM359 |
Partial CRISPR knock-out of IRE1α in PWM253, containing one intact ERN1 allele |
| Cell line (Homo-sapiens) |
U-2 OS IRE1a-HaloTag endogenously tagged |
This paper |
PWM360 |
Introduction of a C-terminal HaloTag into the endogenous ERN1 locus of PWM359 cells. Clonal population. |
| Cell line (Homo-sapiens) |
U-2 OS IRE1a-HaloTag endogenously tagged, low expression clone |
This paper |
PWM361 |
Introduction of a C-terminal HaloTag into the endogenous ERN1 locus of PWM359 cells. clonal population with lower IRE1 expression level than PWM360. |
| Recombinant DNA reagent (plasmid) |
SpCas9 and gRNA targeting the C-terminus of HsIRE11 |
This paper |
pPW3754 |
Used for endogenous CRISPR editing of ERN1 gene. |
| Recombinant DNA reagent (plasmid) |
HDR-HsIRE1a-10xGS-HaloTag |
This paper |
pPW3755 |
Used for endogenous CRISPR editing of ERN1 gene. |
| Recombinant DNA reagent (plasmid) |
CMVd3-ERmembrane-HaloTag-KKMP |
This paper |
pPW3756 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-ERmembrane-2xHaloTag-KKMP |
This paper |
pPW3757 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-HsIRE1-HaloTag |
This paper |
pPW3758 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-HsIRE1deltaLD-HaloTag |
This paper |
pPW3759 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-HsIRE1-K599A_KinaseDead-HaloTag |
This paper |
pPW3760 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag |
This paper |
pPW3761 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-HsIRE1(K121Y)-HaloTag |
This paper |
pPW3762 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-HsIRE1dLKR-HaloTag |
This paper |
pPW3763 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-ERmembrane-GST-HaloTag-KKMP |
This paper |
pPW3781 |
Used for transient transfections |
| Recombinant DNA reagent (plasmid) |
CMVd3-ERmembrane-GST-2xHaloTag-KKMP |
This paper |
pPW3783 |
Used for transient transfections |
| Antibody |
Anti- IRE1α (Rabbit monoclonal) |
Cell Signaling Technology |
3294 |
WB (1:1000) |
| Antibody |
Anti- PERK (Rabbit monoclonal) |
Cell Signaling Technology |
3192 |
WB (1:1000) |
| Antibody |
Anti- ATF4 (Rabbit monoclonal) |
Cell Signaling Technology |
11,815 |
WB (1:1000) |
| Antibody |
Anti- CHOP (Mouse monoclonal) |
Cell Signaling Technology |
2895 |
WB (1:1000) |
| Antibody |
Anti- ATF6 (Mouse monoclonal) |
Proteintech |
66563–1 |
WB (1:1000) |
| Antibody |
Anti- β-actin (Rabbit monoclonal) |
Cell Signaling Technology |
5125 |
WB (1:1000) |
| Antibody |
Anti-IRE1p (Rabbit monoclonal) |
Chang et al., 2018
|
N/A |
WB (1:1000) |
| Antibody |
Anti-XBP1s (Rabbit monoclonal) |
Chang et al., 2018
|
N/A |
WB (1:1000) |
| Sequence-based reagent |
Hs00176385_m1 |
ThermoFisher Scientific |
|
qPCR primer for IRE1 |
| Sequence-based reagent |
Hs02856596_m1 |
ThermoFisher Scientific |
|
qPCR primer for XBP1u |
| Sequence-based reagent |
Hs03929085_g1 |
ThermoFisher Scientific |
|
qPCR primer for XBP1s |
| Sequence-based reagent |
Hs01045913_m1 |
ThermoFisher Scientific |
|
qPCR primer for DGAT2 |
| Sequence-based reagent |
Hs00170663_m1 |
ThermoFisher Scientific |
|
qPCR primer for BCAM |
| Sequence-based reagent |
Hs00197728_m1 |
ThermoFisher Scientific |
|
qPCR primer for TGOLN2 |
| Sequence-based reagent |
s02800695_m1 |
ThermoFisher Scientific |
|
qPCR primer for HPRT1 |
| Commercial assay or kit |
TaqMan RNA-to-CT 1-Step Kit |
ThermoFisher Scientific |
4392938 |
|
| Commercial assay or kit |
RNeasy Plus kit |
Qiagen |
74,134 |
|
| Commercial assay or kit |
In-Fusion HD Cloning |
Clontech |
Clontech:639,647 |
|
| Software, algorithm |
saSPT |
Heckert et al., 2021
|
https://github.com/alecheckert/saspt
|
Open-source software package used for extracting diffusion coefficients from single-particle trajectories. |
| Other |
JF549 dye conjugated with HaloTag ligand |
Luke Lavis Lab; Grimm et al., 2017
|
Promega: GA1110 |
Kind gift of Luke Lavis; also available commercially from Promega |
| Other |
JF646 dye conjugated with HaloTag ligand |
Luke Lavis Lab; Grimm et al., 2017
|
Promega: GA1120 |
Kind gift of Luke Lavis; also available commercially from Promega |
