FIG. 3.

(A) Northern analysis of the ald1 gene. A 20-μg portion of total RNA was loaded on each lane, and ald1 transcription was detected by hybridization with the ³²P-labeled ald1 fragment as a probe. RNA was prepared from Acinetobacter sp. strain M-1 cells grown on a salt medium containing 1% (wt/vol) sodium acetate (NaAc) or 0.5% (vol/vol) n-alkane, the carbon length of which is indicated. (B) Relative enzyme activity of aldehyde dehydrogenase in cells grown on each substrate. The enzyme activity was measured under standard conditions using tetradecanal as a substrate. One hundred percent corresponds to 0.76 U/mg of protein.