Table 1. Characteristics of lab-developed direct RT-qPCR compared to conventional RT-qPCR.

(a) Agreement of the conventional RT-qPCR and lab-developed RT-qPCR
Lab-developed RT-qPCR	Conventional RT-qPCR
	Positive	Negative	Total
Positive	111	0	111
Negative	5	654	659
Total	116	654	770
(b) The qualitative results of conventional RT-qPCR and lab-developed RT-qPCR by agreement statistics
Agreement Coefficient	0.974 (CI = 0.952–0.994)
Accuracy	99.35% (CI = 99.49–99.68%)
Sensitivity	95.69% (CI = 90.23–98.59%)
Specificity	100.00% (CI = 99.44–100.00%)
Positive predictive value	100%
Negative predictive value	99.24% (CI = 98.23–99.68%)
AUC ratio	0.978 (CI = 0.957–1.000)
McNemar’s Test (p-value)	0.65 (p < 0.001)