. 2022 Jun 2;14(11):4624–4633. doi: 10.18632/aging.204095

Table 2. Assessment of limit of detection for lab-developed direct RT-qPCR on the real-time PCR machine.

Real-time PCR machine	Gene target/fluorescent dye	No. of replicates detected as positive / No. of replicates tested at indicated of RNA control copies per reaction (percentage)
Real-time PCR machine	Gene target/fluorescent dye	240	120	60	30	15	7.5
LightCycler^® 96	N1/FAM	10/10 (100)	20/20 (100)	20/20 (100)	19/20 (95)	17/20 (85)	10/20 (50)
LightCycler^® 96	E/HEX	10/10 (100)	20/20 (100)	20/20 (100)	17/20 (85)	12/20 (60)	3/20 (15)
LabTurbo AIO	N1/FAM	10/10 (100)	20/20 (100)	20/20 (100)	19/20 (95)	16/20 (80)	11/20 (55)
LabTurbo AIO	E/HEX	10/10 (100)	20/20 (100)	20/20 (100)	16/20 (80)	12/20 (60)	3/20 (15)