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Sensitivity and specificity of antizearalenone scFv antibodies measured by CI-ELISA using ZEN-OVA-coated wells. Bound soluble scFv antibody was detected by mouse anti-E tag antibody followed by horseradish-peroxidase-conjugated goat anti-mouse IgG. EY.5HL3 was produced in E. coli and plantibody was produced in Arabidopsis.
