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SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of antizearalenone scFv plantibody expression. (A) An SDS-PAGE gel was stained with Coomassie brilliant blue G-250. (B) Proteins in a duplicate SDS-PAGE gel were detected by anti-E tag antibody. Lanes 1, soluble proteins of Arabidopsis from untransformed plants; lanes 2, vector pKYLX71::35S2-transformed plants; lanes 3, pQY4.4-1-transformed plants; lane M, protein molecular size marker with a 10-kDa ladder.
