TABLE 1.
Effect of pH environment on antibody binding activitya
| Antizearalenone antibody | Relative binding activity (%)b
|
||
|---|---|---|---|
| pH 6.3 | pH 2.3 | pH 2.3 then pH 6.3 | |
| MAb | 100 | 0 | 99 |
| Bacterial scFv | 100 | 0 | 98 |
| scFv plantibody | 100 | 0 | 89 |
a
Antibodies were diluted (1:5 [vol/vol]) in pH 2.3 or pH 6.3 buffer solutions (adjusted with 0.05 M citric acid or 0.05 M Na2HPO4) and incubated at 4°C overnight. A portion of the pH 2.3-treated sample was adjusted back to pH 6.3 and incubated at room temperature for 1 h.
b
Binding activity at pH 6.3 was set at 100%.