TABLE 2.

Intensity of gold labeling of antizearalenone scFv associated with different components of plant cells in transgenic plants expressing antizearalenone scFv and in nontransformed healthy plants^a

Cell component	No. of gold particles/μm2 (mean ± SE)
	Transgenic plant	Healthy plant
Chloroplast	189 ± 3.1	3.6 ± 0.55
Nucleus	187 ± 3.6	4.6 ± 0.70
Cytoplasm	191 ± 2.5	2.6 ± 0.80
Vacuole	1.9 ± 0.56	2.3 ± 0.61
Rough endoplasmic reticulum	194 ± 3.0	3.2 ± 0.85
Cell wall	172 ± 2.0	2.4 ± 0.89
Intercellular space	23 ± 2.1	2.8 ± 0.7

^aScFv was detected using mouse anti-E tag antibody followed by goat anti-mouse IgG-gold conjugate. Untransformed plant leaf sections were treated with antizearalenone scFv followed by goat anti-mouse IgG-gold conjugate.