Figure 4.

CLU was directly inhibited by miR-1276 and up-regulated in OC patients. (A) The binding site of CLU-WT or -Mut for miR-1276 was shown. (B) Luciferase reporter assay showed that CLU was a target gene of miR-1276. (C) miR-1276 up-regulation decreased CLU expression, and miR-1276 down-regulation increased CLU expression. (D) circ_0063804 down-regulation reduced CLU expression, and circ_0063804 up-regulation elevated CLU expression. (E) CLU expression was dramatically increased in tumor tissues than that in normal tissues of OC patients. (F) In OC tissues, CLU expression level was positively correlated with circ_0063804 expression, and negatively correlated with miR-1276 expression. ** P < 0.01. OC: ovarian cancer.