FIG. 3.

Detection of the V. hollisae gyrB gene by PCR. Primer pair HG-F1 and HG-R2 and the amplification conditions and gel electrophoresis method described in Materials and Methods were employed. Lanes: 1, molecular weight markers (φX 174 phage DNA digested with HaeIII); 2 to 9, V. hollisae strains 89A 1961, 89A 1960, 89A 4206, 91A 2120, 93A 5688, SJ 90, FO 93, and RIMD 2221011, respectively; 10, V. anguillarum PT-87050; 11, V. cholerae O1 NIH41; 12, V. fluvialis RIMD 2220002; 13, V. furnisii RIMD 2223001; 14, V. parahaemolyticus WP1; 15, V. vulnificus RIMD 2219009; 16, V. alginolyticus AM2; 17, V. damsela RIMD 2222001. The position of the specific amplicons (363 bp) is indicated by the solid triangle.