TABLE 1.
Results of the tests for the gyrB and toxR genes
| Species | Sourcea or strain no. | Result of detectionb by:
|
|||
|---|---|---|---|---|---|
| DNA probec
|
PCR
|
||||
| gyrB gene | toxR gene | gyrB gened | toxR genee | ||
| Vibrio hollisae | C | + (11/11) | + (11/11) | + (11/11) | + (11/11) |
| RIMD 2221011f | + | + | + | + | |
| Vibrio cholerae O1 | C | +W(2/2) | − (0/2) | − (0/2) | − (0/2) |
| Vibrio cholerae O1 | NIH 35A3 | +W | − | − | − |
| Vibrio cholerae O139 | MO45 | +W | − | − | − |
| Vibrio cholerae non-O1, non-O139 | AM2 | +W | − | − | − |
| Vibrio parahaemolyticus | C | +W(4/4) | − (0/4) | − (0/4) | − (0/4) |
| Vibrio fluvialis | C | +W(2/2) | − (0/2) | − (0/2) | − (0/2) |
| Vibrio furnisii | RIMD 2223001 | +W | − | − | − |
| Vibrio damsela | C | +W(2/2) | − (0/2) | − (0/2) | − (0/2) |
| Vibrio metschnikovii | C | − (0/2) | − (0/2) | − (0/2) | − (0/2) |
| Vibrio mimicus | RIMD 2218001 | +W | − | − | − |
| Vibrio vulnificus | RIMD 2219009 | +W | − | − | − |
| Vibrio alginolyticus | E | +W(3/3) | − (0/3) | − (0/3) | − (0/3) |
| Vibrio cincinnatiensis | ATCC 35912 | +W | − | − | − |
| Vibrio carchariae | ATCC 35084 | +W | − | − | − |
| Vibrio anguillarum | PT-87050 | − | − | − | − |
| Vibrio ordalii | ATCC 33509 | +W | − | − | − |
| Vibrio campbellii | ATCC 25920 | +W | − | − | − |
| Vibrio harveyi | ATCC 14126 | +W | − | − | − |
| Vibrio nereis | ATCC 25917 | +W | − | − | − |
| Vibrio pelagius | ATCC 25916 | +W | − | − | − |
| Vibrio splendidus | ATCC 33125 | +W | − | − | − |
| Vibrio tubiashii | ATCC 19109 | − | − | − | − |
| Vibrio aestuarianus | ATCC 35048 | − | − | − | − |
| Vibrio mediterranei | ATCC 43341 | +W | − | − | − |
| Vibrio orientalis | ATCC 33934 | +W | − | − | − |
| Vibrio diazotrophicus | ATCC 33466 | +W | − | − | − |
| Vibrio gazogenes | ATCC 29988 | +W | − | − | − |
| Vibrio proteolyticus | NCMB 1326 | +W | − | − | − |
| Vibrio logei | ATCC 15382 | +W | − | − | − |
| Vibrio nigripulchritudo | ATCC 27043 | − | − | − | − |
| Vibrio navarrensis | ATCC 51183 | +W | − | − | − |
| Vibrio mytili | ATCC 51288 | − | − | − | − |
| Vibrio iliopiscarius | HUFP 9601 | +W | − | − | − |
| Vibrio ichthyoenteri | IFO 15847 | +W | − | − | − |
| Vibrio penaeicida | IFO 15640 | − | − | − | − |
| Unknown (non-Vibrio hollisae)g | E | NTh | − (0/74) | − (0/74) | − (0/74) |
| Escherichia coli | MC1061 | +W | − | − | − |
a
C, clinical source; E, environmental source.
b
+, detected; +W, weak reaction detected; −, not detected. For species of which multiple strains were tested, the number of positive strains/the number of tested strains is given in parentheses.
c
DNA probes specific to the V. hollisae gyrB and V. hollisae toxR genes (described in the text) were used.
d
Results obtained when primer pairs HG-F1 and HG-R2, HG-F2 and HG-R1, and HG-F2 and HG-R2 were employed.
e
Results obtained when primer pair HT-F3 and HT-R2 was employed.
f
Same as KUMA871, an isolate from a coastal fish (19).
g
Seventy-four strains isolated from marine fish (explained in the text).
h
NT, not tested.