TABLE 4.
Comparison of DNA extraction methods for the 35-cycle PCR to detect V. hollisae 525-82SmR after a 4-h enrichment in APW containing a seafood sample
| Seafood samplea | DNA extraction methodb | Lowest initial level (CFU/ml) of 525-82SmR detectable by PCR |
|---|---|---|
| Oyster | 1 | 102 |
| 2 | 1 | |
| 3 | 1 | |
| Octopus | 1 | 103 |
| 2 | 103 | |
| 3 | 10 | |
| Shrimp | 1 | 104 |
| 2 | 104 | |
| 3 | 102 |
a
See the footnotes c, f, and h of Table 3 for details.
b
1, boiling; 2, chaotropic extraction followed by ethanol precipitation; 3, chaotropic extraction followed by absorption onto silica-coated magnetic beads.