Fig. 1.

Carns1^−/− mice. A Schematic presentation of the wild type Carns1 gene and the Carns1 knock-out allele in Carns1^−/− mice analysed in this study. P/F indicate position of loxP and FRT sites that remain after cre mediated recombination in the knockout allele. All coding exons are deleted. B Western blot analysis (10% SDS-PAGE; 20 µg protein/lane) of skeletal muscle confirmed absence of Carns1 protein in Carns1^−/− mice. C Kaplan–Meier curve showed no significant difference between genotypes in survival rate