Fig. 5.

Analysis of MDA-adducts and anti-oxidant enzymes in Lepr^db/db/Carns1^−/− mice. A Western blot analysis (10% SDS-PAGE, 20 µg protein/lane) of MDA adducts in kidneys from Lepr^db/db/Carns1^−/− mice. B Densitometric quantification of MDA Western blots (mean ± SD, n = 4 mice per genotype). *p < 0.05; ns, not significant. C Western blot analysis (12.5% SDS-PAGE; 20 µg/lane) of anti-oxidant enzymes GSR, SOD1 and SOD2 in kidneys from 5-month-old mice (genotypes as indicated). D Densitometric quantification of GSR, SOD1 and SOD2 (mean ± SD, n = 4 mice per genotype). No significant differences were observed