Table 1.

Protein carbonyl content in brain, kidney and skeletal muscle

Tissue	Age (months)	Carns1+/+	Carns1−/−	Two-way ANOVA
Brain	18	1.14 ± 0.22 (n = 3)	1.10 ± 0.19 (n = 3)	Effect of age (p < 0.001)
	24	1.93 ± 0.22 (n = 4)	1.82 ± 0.16 (n = 4)
Kidney	18	0.76 ± 0.14 (n = 3)	0.98 ± 0.49 (n = 3)	Effect of age (p < 0.001)
	24	1.66 ± 0.36 (n = 4)	1.76 ± 0.24 (n = 4)
Skeletal muscle	18	1.60 ± 0.63 (n = 6)	2.18 ± 0.42 (n = 6)	No significant effect
	24	1.62 ± 0.19 (n = 4)	1.79 ± 0.15 (n = 4)

Carbonyl content (nmol/mg protein) in the indicated tissues from 18- and 24-month-old mice was determined spectrophotometrically after derivatization with DNPH, as described in “Materials and methods” (shown are mean ± SD; n = 3–6)