Table 1.
Accuracy (recovery, %) and precision (RSD, %) of the GC–MS method for free hypusine in urine samples from two volunteers
| Hyp added (µM) | Hyp measured (µM) | Recovery (%) | RSD (%) |
|---|---|---|---|
| Urine #1 | |||
| 0 | 0.593 ± 0.011 | Not applicable | 1.95 |
| 1 | 1.463 ± 0.050 | 87.0 | 3.44 |
| 2 | 2.503 ± 0.050 | 95.5 | 2.01 |
| 3 | 3.417 ± 0.244 | 94.1 | 7.15 |
| 4 | 4.357 ± 0.040 | 94.1 | 2.72 |
| 5 | 5.170 ± 0.335 | 91.5 | 3.42 |
| Urine #2 | |||
| 0 | 1.890 ± 0.078 | Not applicable | 3.44 |
| 1 | 2.680 ± 0.122 | 79.0 | 4.59 |
| 2 | 3.643 ± 0.211 | 87.7 | 5.79 |
| 3 | 4.597 ± 0.040 | 90.2 | 0.88 |
| 4 | 5.557 ± 0.033 | 91.7 | 6.03 |
| 5 | 6.043 ± 0.216 | 83.6 | 3.57 |
Urine samples #1 and #2 were analyzed in parallel and in triplicate