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. 2022 Jun 22;12:10521. doi: 10.1038/s41598-022-13804-y

Table 1.

Identification of Bacillus strains used through 16S rRNA, sbo and biochemical characterization through CHB-50 PCR of. Subtilosin A production was also noted for correlation.

S.n. and Strain designationa Source Homology 16S r RNA Sbo PCR detectionb Final Bacterial identification Subtilosin A productionc
1. B. subtilis 168 BGSC B. subtilis subsp. subtilis 168 +I B. subtilis subsp. subtilis 168 +
2. B. subtilis SOIL B. subtilis subsp. Subtilis +I B. subtilis subsp. subtilis +
3. B. subtilis SOIL B. subtilis subsp. Subtilis +I B. subtilis subsp. subtilis +
4. B. subtilis SOIL B. subtilis strain CICC148 +I B. subtilis subsp. subtilis +
5. B. subtilis SOIL B. subtilis subsp. Subtilis +I B. subtilis subsp. subtilis +
6. B. subtilis SOIL/WATER B. subtilis subsp. Subtilis +I B. subtilis subsp. subtilis +
7. B. subtilis SOIL/WATER B. subtilis subsp. Subtilis +I B. subtilis subsp. subtilis +
8. B. subtilis SOIL B. polyfermenticus +I B. subtilis subsp. subtilis + 
9. B. subtilis SOIL B. subtilis subsp. Subtilis +I B. subtilis subsp. subtilis +
10. B. fusiformis SOIL B. sp .strain MHS006 – B. subtilis subsp. subtilis –
11. B. subtilis 7 mut (-A) SOIL B. subtilis subsp. Subtilis – B. subtilis subsp. subtilis –
12. B. subtilis ATCC6633 BGSC B. subtilis subsp. subtilis ATCC6633 +I B. subtilis subsp. subtilis ATCC6633  + 
13. B. subtilis SOIL/DRY B. subtilis subsp. Spizezinii +II B. subtilis subsp. spizezinii  + 
14. B. subtilis SOIL/DRY B. subtilis subsp. Spizezinii +II B. subtilis subsp. spizezinii +
15. B. mojavensis SOIL/DRY B. subtilis strain Au53 +II B. subtilis subsp. spizezinii +
16. B. mojavensis SOIL B. mojavensis +I B. subtilis +
17. B. licheniformis SOIL B. mojavensis – B. licheniformis –
18. B. licheniformis SOIL B. licheniformis – B. licheniformis –
19. B. licheniformis SOIL B. licheniformis – B. licheniformis –
20. B. licheniformis SOIL B. licheniformis – B. licheniformis –
21. B. licheniformis SOIL B. sp. Ni 21 – B. licheniformis –
22. B. licheniformis SOIL B. amyloliquefaciens – B. licheniformis –
23. B. licheniformis SOIL B. amyloliquefaciens – B. licheniformis –
24. B. licheniformis SOIL B. licheniformis strain KL-176 – B. licheniformis –
25. B. licheniformis SOIL/DRY B. amyloliquefaciens – B. licheniformis –
26. B. amyloliquefaciens SOIL/DRY B. amyloliquefaciens  + I B. amyloliquefaciens  + 
27. B. amyloliquefaciens SOIL B. amyloliquefaciens  + I B. amyloliquefaciens  + 
28. B. amyloliquefaciens SOIL B. amyloliquefaciens – B. licheniformis –
29. B. amyloliquefaciens SOIL/DRY B. fusiformis – B. licheniformis –
30. B. pumilus SOIL B. pumilus – B. licheniformis –
31. B. amyloliquefaciens SOIL/WATER B. fusiformis – B. licheniformis –

aIdentification by using CHB-50.

b+ PCR product of the expected size was detected. I and II assigned for subsp. subtilis and spizizenii respectively.