Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 22;12:10568. doi: 10.1038/s41598-022-14933-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Crown 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

In vitro kinase assay validation of hnRNPM phosphorylation by MASTL. (a) The ion abundance of each phospho-site activated in triplicate reactions between MASTL kinase and hnRNPM (as detailed in this figure legend). Phospho-site abundances were calculated using label-free quantitation. Square brackets indicate peptide locations within the protein on which each phospho-site is found. Single hashtags denote a co-modification within that peptide, e.g., carbamidomethylation, deamidation, and/or oxidation. Double hashtags denote a different combination of co-modifications within the same peptide (detailed in Supplementary Table S7). Error bars display the standard error of the mean from three (n = 3) independent reactions. (b) Schematic of hnRNPM showing nuclear localisation domain (NLS) RNA recognition motifs (RRM) and phospho-sites currently annotated in PhosphoSitePlus (Chk1 and PRP4) along with the novel MASTL sites identified here. Sequence alignments of the MASTL phosphorylation sites within hnRNPM (S-633 and S-637), compared to validated substrates Arpp19/ENSA and the possible MASTL motif identified from our SILAC screen (Fig. 2d).