Figure 1.

Schematic overview of the isolation and culture conditions of chicken intestinal organoid. (1) Isolation of small and large intestines (2) Intestinal tissue was cut into tiny segments and washed with PBS-EDTA. (3) To obtain a homogenous suspension, segments were minced using a Potter–Elvehjem PTFE pestle and glass tube. (4) After multiple centrifugation steps, the pellet was resuspended in ice-cold Matrigel (5) and seeded in a culture plate (6). After polymerization of Matrigel, crypts were cultured with indicated media as defined in supplementary Table 1. (7) Monolayer cultures were obtained from three-dimensional cultures.