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. 2022 Jun 22;12:10563. doi: 10.1038/s41598-022-14875-7

Figure 4.

Figure 4

Supplementary compounds for organoid growth. Brightfield images of embryonic chicken organoid cultured in Matrigel with BCM of passage 1, 1,2 and 3 days after splitting. (a,e,i) with additional RSPO1 and WNT3 of chicken origin. (b,f,j) with additional RSPO1/WNT3 of chicken origin and 0.2 µM Forkhead box O1-inhibitor (FOXO1-i). (c,g,k) with additional RSPO1/WNT3 and 2.5 µg/ml prostaglandin E2 (PGE2) (d,h,l). With all 4 supplemented growth factors (WNT3/RSPO1/PGE2/FoxO1-i). Images are representative of three independent cultures. Scale bar: 1000 µm. (m) Diameter of organoids when culturing with different supplements, were measured manually and plotted in Graphpad Prism. Four outliers were excluded after performing an outlier test in Graphpad Prism. Statistics were performed using a Kruskal–Wallis test followed by Dunn’s multiple comparisons test. *p < 0.05 **p < 0.01.