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. 2022 Jun 22;12:10563. doi: 10.1038/s41598-022-14875-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Longevity of the chicken intestinal organoids. (a) Brightfield images of chicken intestinal organoids cultured in chicken organoid culture medium. All pictures were taken 2 days after passaging. Scale bar: 400 µm. (b) mRNA levels of LGR5 in 3D embryonic chicken intestinal organoids, expressed as 40-Ct values with GAPDH as a reference gene. Two independent isolations were performed in technical triplicates. The error bars represent the standard deviation of the mean.