Table 1.
Characteristic of the used antibodies.
| Antibody | Concentration | Species cross-reactivity | Target site | Host/Isotype |
|---|---|---|---|---|
|
Anti-Sox9 AB5535 Sigma-Aldrich |
1:500 | Human, Mouse, Rat, Chicken | Sox9, a transcription factor and recognized marker for stem/progenitor cells | Rabbit |
|
Anti-chromogranin-A Immunostar 20086 |
1:500 | Buffalo, Chicken, Cow, Dolphin, Human, Mouse | Enteroendocrine cells | Rabbit |
|
Anti-occludin Thermofisher 71-1500 |
1:100 | Baboon, Bacteria, Chicken, Dog, Human, Mouse, Rabbit, Rat | Tight junction membrane | Rabbit |
| Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | 1:1000 | Rabbit | Gamma Immunoglobins Heavy and Light chains | Donkey/IgG |