Table 1.
Sequences of the analyzed hammerhead ribozymes
| Names | Sequences of the hammerhead ribozymes | Target ((+)RNA numbering) | Cleavage NUH triplet |
|---|---|---|---|
| RH1NL | 5'-UAAUCACUCUGAUGAGUCCGUGAGGACGAAAGUUUGAG-3' | 184–200 | CUC |
| RH1 | 5'-UAAUCACUCUGAUGAGUCCGUGAGGACGAAAGUUUGAGL-3' | ||
| RH2 | 5'-AUCACUCUGAUGAGUCCGUGAGGACGAAAGUUUGAG-3' | 184–198 | |
| RH3 | 5'-UAAUCACUCUUAUGAGUCCGUGAGGACGAAAGUUUGAG-3' | 184–200 | |
| RB1 | 5'-AUCACUCUGAUGAGGCCGAAAGGCCGAAAGUUUG-3' | 186–198 | |
| RB2 | 5'-CCUAAGGCCAAAGCUAUGGAUCACUCAGAUGCGGCCGAAAGGCCGUAAGUUUG-3' | ||
| RB3 | 5'-AUCACUCUUAUGAGGCCGAAAGGCCGAAAGUUUG-3' | ||
| RZ6A | 5'-UUCUGAUUCUGAUGAGUCCGUGAGGACGAAACUCCAUUL-3' | 615–631 | GUU |
| RZ6C | 5'-CCACGUUUCUGAUGAGUCCGUGAGGACGAAAUCAUUCUL-3' | 628–644 | AUC |
| RZ6D | 5'-UCAUAAGCCUGAUGAGUCCGUGAGGACGAAACCCUUGUL-3' | 688–704 | GUU |
The sequence of the hammerhead ribozyme catalytic core is marked as bold letters, and its mutations are indicated in italic. The TLR motif is underlined in the ribozyme sequence. The LNA residue is marked with NL.